LOCUS       aacC2-tmrB_region       7829 bp    DNA     linear       06-OCT-2020
DEFINITION  Enterobacter cloacae.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Unclassified.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of aacC2-tmrB region
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
     repeat_region   complement(1..14)
                     /gene="IRR_IS26"
     CDS             complement(53..757)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYISSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   complement(807..820)
                     /gene="IRL_IS26"
     repeat_region   918..955
                     /gene="IRR_Tn2"
     CDS             990..1850
                     /gene="aacC2"
                     /codon_start=1
                     /product="Aminoglycoside-3-N-acetyltransferase"
                     /translation="MHTRKAITEALQKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDEARRTWLPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRDGEVAWKTASDYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGTTPIVPPHE
                     AVERSCEPSG"
     CDS             1863..2405
                     /gene="tmrB"
                     /codon_start=1
                     /product="Tunicamycin resistance protein"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVP
                     MPASGDYQDLPLWRGLTIAAVREIRRNYSQDIIIPMTLVHPDYLTEILDGVRRIDDQL
                     LHIFLTLNEDLLRHRIANQTMHPDPNRNAEIREWRLANVARCLAARERLPCTTRVLDS
                     GAHTSDELAAMVLDGIDGRT"
     CDS             complement(2887..3078)
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MAIALMGAGFSATDTSDAVNILYPITMTVQANKAWQASGLKKSF
                     FLPSHKKVRGGEKISYARL"
     CDS             3102..3329
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MVNSYSLSDDSGVMAAAAITHFLFGQAVFSYLNGWSVLIGPGTG
                     LDSTGCKYARDLMGLVAFTAFIVTFLFRGYS"
     CDS             3359..4516
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MTVRKDPVSQLSQLRSPAAVQAAIDEFVQLGRTKFLARHGYGKS
                     RDFLVRDPKTGTDCDSKAIAGVAFGKQFPEQGPLTADSFSGGETTVVPALTRLGFRII
                     RIGEDWSEEEVLATVEDYFDMLRAEAAGEPYHKSEHNQALRQLLNGRSKSSVELKHQN
                     ISAVLDALGLPYINGYKPRGNSQLLLRKSVHAYVLEHQQTVGALVDALEEVKLPGDKT
                     YRAALVEPPAREVLVRTPASLRQRLPRKFDYAARDEANRKLGRAGEQWVIGYEQQRLT
                     ELGHPELFQRLDWVSDTQGDGAGFDILSFEEDAHERFIEVKTTNGGVGSSFLVSHNEL
                     EFSKEAGDQFHLYRVFQFRDGPRLFTLPGDLSQHVHLKPTDYRASFRSLVG"
     repeat_region   4550..4574
                     /gene="IRL_ISCfr1"
     CDS             4631..6001
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISCfr1 transposase"
                     /translation="MMGQLPGGQQRLFYSFNLEDHVPAQHLLRSIDQCLDLSDLRAYL
                     ADFYSPIGRPSIDPELMVRMLVVGYCYGIRSERRLCEEVHLNLAYRWFCRLGLEDEVP
                     NHSTFSKNRHGRFRDSDLFRWLFNEVLRRCMAAGLVKGEGFAVDASIIKADASRQRGV
                     AGDEVDWNDPKLSSRAVREYLEALDEEALAEALPKKISLTDPQSRWTAAPGGPAFFAY
                     STNYLIDTEHGVIMDVEATPAHRTAEVDSTRTMVERVEAQFDLTPERLIGDTAYGTAP
                     MLAWMVEEKDIEPHVPVWDKTERKDDSLSSNDFHWSQDANEYRCPAGKPLRSEWRAFT
                     QQRSRVTKAKTVIYRSSQTDCATCPLKAKCCPNTPNRKIVRSIHEAARDVARRIAKTP
                     EYLVSRCERKKVEMLFAHLKRIMKLDRLRLRGLTGATDEFTLAAMVQNLRRMAKLLPQ
                     GPPLTG"
     repeat_region   6142..6166
                     /gene="IRR_ISCfr1"
     CDS             6822..7682
                     /gene="blaTEM-1"
                     /codon_start=1
                     /product="Beta-lactamase TEM-1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     repeat_region   7792..7829
                     /gene="IRR_Tn2"
ORIGIN      
        1 ggcactgttg caaagttagc gatgaggcag ccttttgtct tattcaaagg ccttacattt
       61 caaaaactct gcttaccagg cgcatttcgc ccaggggatc accataataa aatgctgagg
      121 cctggccttt gcgtagtgca cgcatcacct caataccttt gatggtggcg taagccgtct
      181 tcatggattt aaatcccagc gtggcgccga ttatccgttt cagtttgcca tgatcgcatt
      241 caatcacgtt gttccggtac ttaatctgtc ggtgttcaac gtcagacggg caccggcctt
      301 cgcgtttgag cagagcaagc gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc
      361 gcgggatctg ccacttcttc acgttgttga ggattttacc cagaaaccgg tatgcagctt
      421 tgctgttacg acgggaggag atataaaaat cgacagtgcg gccccggctg tcgacggccc
      481 ggtacagata cgcccagcgg ccattgacct tcacgtaggt ttcatccatg tgccacgggc
      541 aaagatcgga agggttacgc cagtaccagc gcagccgttt ttccatttca ggcgcataac
      601 gctgaaccca gcggtaaatc gtggagtgat cgacattcac tccgcgttca gccagcatct
      661 cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga
      721 tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg
      781 gatgataagt ttatcaccac cgactatttg caacagtgcc gtttactcat atatacttta
      841 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      901 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtata
      961 gtgttttgca gtttagagga gatatcgcga tgcatacgcg gaaggcaata acggaggcgc
     1021 ttcaaaaact cggagtccaa accggtgacc tcttgatggt gcatgcctca cttaaagcga
     1081 ttggtccggt cgaaggagga gcggagacgg tcgttgccgc gttacgctcc gcggttgggc
     1141 cgactggcac tgtgatggga tacgcgtcgt gggaccgatc accctacgag gagactctga
     1201 atggcgctcg gctggatgac gaagcccgcc gtacctggct gccgttcgat cccgcaacag
     1261 ccgggactta ccgtgggttc ggcctgctga atcaatttct ggttcaagcc cccggcgcgc
     1321 ggcgcagcgc gcaccccgat gcatcgatgg tcgcggttgg tccgctggct gaaacgctga
     1381 cggagcctca cgaactcggt cacgccttgg gggaaggatc gcccgtcgag cggttcgttc
     1441 gccttggcgg gaaggccctg ctgttgggtg cgccgctaaa ctccgttacc gcattgcact
     1501 acgccgaggc ggttgccgat atccccaaca aacggtgggt gacgtatgag atgccgatgc
     1561 ttggaagaga cggtgaagtc gcctggaaaa cggcatcgga ttacgattca aacggcattc
     1621 tcgattgctt tgctatcgaa ggaaagccgg atgcggttga aactatagca aatgcttacg
     1681 tgaagctcgg tcgccatcga gaaggtgtcg tgggctttgc tcagtgctac ctgttcgacg
     1741 cgcaggacat cgtgacgttc ggcgtcacct atcttgagaa gcatttcgga accactccga
     1801 tcgtgcctcc gcacgaggcc gtcgagcgct cttgcgagcc ttcaggttag aggccgtcga
     1861 caatgataat ctggatcaac ggacctttcg gcgccggaaa gacgacgctc gctaagcggc
     1921 tgcgcgatcg gcgttccaaa tcgctgatct ttgaccccga ggaaatcggg ttcgtggtga
     1981 aagaaacggt ccccatgcca gcgagcggag actatcagga tctccccttg tggaggggac
     2041 ttacgatcgc ggcggtcagg gagattcgaa ggaattactc gcaggacatc atcatcccaa
     2101 tgacgctcgt gcacccggac tatctgactg agatactcga cggggtaagg cggatcgacg
     2161 atcagctgct gcacatcttt ctgacgctca acgaggacct attgcgtcac cggatcgcga
     2221 accagaccat gcatcctgac ccgaatcgaa atgcggagat tcgagagtgg cgattagcga
     2281 atgtcgcccg atgcttggcc gcaagggaac ggcttccatg cacaacccgt gttctcgata
     2341 gtggtgcaca caccagcgat gaactcgcag cgatggtgct cgacggaatc gatgggcgca
     2401 cctgatcgcc ttcgacgcct gcgcaaagcg tagcgcgagg gtggcgggct cacgaccaaa
     2461 cgcccagagg tcgatcatcg cagggatgtt tggctttgtg gtgcggacga cgggactcga
     2521 acccgtactc tcacagagaa gcagattttc gtaccacctc gactttcgcc gccgtctgat
     2581 gacgttcgtg gtctggactg tcccttcgcc attgcccgaa ggctttaggc gccgcccgtc
     2641 cagtctctac accttccccc gaaggggctt ggctcgggat tggcttaggg tattgcccgt
     2701 tagcgttccc cgactttgag cggttctact ccgcggattt ccccgcgggc actccaattt
     2761 taaagtctgc tgcgtctacc gatttcgcca cgtccgcctt ttttcgccgt tcctagcgct
     2821 cgtgcgatgc acctatgttg cacctagcgc cgaatcgttc ttcgtcatcc tgaaaaacca
     2881 cgtctcctaa agccttgcat agcttatctt ttctccacca cgaacttttt tgtgggatgg
     2941 tagaaaaaaa gactttttaa gtccgctggc ttgccaggcc ttgttagctt gtacggtcat
     3001 ggttatcggg taaagaatat tgacggcatc gctggtgtcg gtggctgaaa agccggctcc
     3061 catcagggca atagccattt cagatgcagg cgtacagggc aatggtcaac agctacagcc
     3121 tgtctgacga ttccggcgtc atggctgcgg cggctatcac gcattttttg ttcggtcagg
     3181 cggtgttttc gtacctcaat ggttggagcg tgttgatcgg acctggtaca ggtttggaca
     3241 gcacgggctg caaatacgca agggatttaa tgggcctggt ggcgttcacg gcttttatcg
     3301 tgacgtttct gttcaggggc tactcataat ctcgtggctc ggcggttccc ggcacaccat
     3361 gacagtaagg aaggaccctg tgtctcaact ctcccagctt cgaagccccg ccgccgtgca
     3421 ggctgccatc gatgagttcg tgcaactggg ccgcacgaaa ttcctggcgc gccacggcta
     3481 cggcaagtcc cgcgacttcc tggtacgtga tccgaagacc ggcaccgatt gcgattccaa
     3541 ggccatcgcc ggtgtggcct tcggcaagca atttcccgag cagggcccgc tcactgctga
     3601 cagcttctcc ggtggcgaga cgaccgtcgt tccggcgctg acgcggctcg ggtttcgcat
     3661 cattcgcatc ggcgaagact ggtccgaaga agaggtcctg gccacggtcg aagactattt
     3721 cgacatgctg cgtgccgagg cggctgggga gccgtaccac aagtccgagc acaaccaggc
     3781 actgcgccaa ctgctgaacg gtcgcagcaa gtcttcagtc gagctcaagc accagaacat
     3841 tagcgccgta ctcgatgccc tgggcctgcc ctatatcaac ggctacaagc cacgcggcaa
     3901 cagccaactg ctgctgcgta aatccgtaca cgcctacgtt ctggaacatc agcagacggt
     3961 cggcgctctt gtcgatgccc tggaggaggt aaaacttccg ggtgacaaaa cctaccgagc
     4021 ggctttggta gaaccacccg cccgtgaagt gcttgtgcgt accccggcat ctctacggca
     4081 acgcctaccg cgaaagttcg attatgccgc tcgcgatgaa gccaaccgca agctgggccg
     4141 ggcaggggag cagtgggtga ttggctacga acagcaacgc ctgaccgagc tcggccaccc
     4201 agagcttttt cagcggctgg attgggtgtc cgacacccag ggagacggtg cggggttcga
     4261 catcctgtcg ttcgaagagg acgcccatga gcgcttcatc gaggtgaaaa ccaccaatgg
     4321 cggggtaggc tcgtctttct tggtcagcca caacgaactc gaattctcca aggaggcggg
     4381 cgatcaattc catctgtatc gcgtgttcca gtttcgggac ggtccgcgcc tgttcacgct
     4441 acccggcgac ctcagccaac atgtgcatct caagccgacg gactaccggg cgagtttccg
     4501 gagtttggtg gggtaaaggc agggttctgt tgagccgaat ggctgtgtgc ggccgattct
     4561 gttgaaaaag tagcggcctc cccatgccgt tggcaaaatt gctttgtcag cgagcgtggg
     4621 ggcgaacagc atgatgggac agttaccggg aggacagcag cgcctgttct actcgttcaa
     4681 tctggaagat cacgtcccgg cccaacatct cctgcgcagc atcgaccagt gcttggatct
     4741 cagtgatcta cgtgcctacc tggcagattt ctatagcccc atcgggcgtc cctcgattga
     4801 cccggagttg atggtgcgca tgctggtcgt cggctactgc tatggcattc gttccgagcg
     4861 gcgattgtgc gaagaggtgc acctgaacct ggcctatcgc tggttctgcc ggttgggtct
     4921 ggaagacgaa gtccccaatc actcgacctt ctcgaagaat cgccatgggc gttttcgtga
     4981 cagcgatcta ttccgctggt tattcaatga ggtgctgcgg cgctgcatgg cagccggcct
     5041 agtcaagggt gaaggtttcg ccgtcgacgc cagcatcatt aaggcggatg ccagccggca
     5101 acgtggggtg gcgggagatg aggtcgattg gaacgatcca aagctcagca gccgcgcagt
     5161 gcgcgagtac ctcgaagccc ttgatgaaga ggcgctggct gaggctcttc ccaagaaaat
     5221 ttcgctcact gatcctcagt cccgttggac agcagcgcca ggtggcccgg ccttttttgc
     5281 ctactccacg aattacctga tcgacactga gcacggtgtg atcatggacg tggaagctac
     5341 cccggcgcac cgtaccgccg aagtcgattc gactaggacg atggtcgagc gtgtcgaggc
     5401 gcagttcgat ctcacaccgg aacgccttat cggcgatacc gcttatggca ccgccccgat
     5461 gctggcctgg atggtcgaag aaaaggacat cgaaccgcat gtgccggtgt gggacaagac
     5521 cgagcgcaag gacgacagcc tctccagtaa cgactttcac tggagtcagg acgccaatga
     5581 atatcgctgc ccagccggca aaccgctacg cagtgaatgg cgcgccttca cccagcaaag
     5641 gtcgcgggta actaaggcca aaaccgtcat ttaccgctcc agccaaaccg actgcgccac
     5701 ctgcccgttg aaagcgaaat gctgccccaa cacgccgaat cggaagatcg tccgcagcat
     5761 ccatgaggct gcccgcgacg tggctcgacg catcgccaag acaccggagt acctcgtctc
     5821 tcgctgcgaa cgaaagaagg tggagatgct tttcgcccac ctcaaacgga tcatgaaact
     5881 cgaccgttta cgactgcgtg gcctaacggg tgccactgac gaattcacct tggctgcgat
     5941 ggtgcagaac ctgcgccgca tggccaagct tttgcctcaa gggccaccgc tgacgggata
     6001 ggtatgcctg ctacgagcag aaaccctcaa attaaccctt aaacctgagc aaggacgctc
     6061 agtgaaacgc cggaaggcaa cttgaagtgg cttgcagcca cttcgacagc aggcacacct
     6121 gatcggcagg ctgccgctaa agctactttt tcaacagaat cggccgattt gtgccggtcg
     6181 tcaaggaccg cttcggatca ctctcaaccg gatcgcgggc cagctgattt gctaaaaccc
     6241 gcgccaaact caacgaagga tttcgcatga tccgtttttt ttggcgatca cgctcggcct
     6301 gatggttggc tgctcgtcga gttcaagttc gatctgatca gtgagcggta gcgcaagtgg
     6361 atcgcctcag ccaagcagcg ttgttgccgg cggccggaag aaagccttga actagtagcg
     6421 gaaaggatga aactttattt ttacgattct cagcattttc caaggttgag cgaaaattag
     6481 gatgatactt taatgattcc ggcctccatc cccacaaaac gcttgcagat ggcagaattg
     6541 aaataagtca ttgttttaaa tggatttttg caggaattgc tacgttgcag ggtcctgtag
     6601 ttaggatgac actttatttt cctcccacag gcctcgtgat acgcctattt ttataggtta
     6661 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6721 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6781 aaccctggta aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacattttc
     6841 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6901 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6961 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7021 tgagcacttt taaagttctg ctatgtggtg cggtattatc ccgtgttgac gccgggcaag
     7081 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7141 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7201 tgagtgataa cactgctgcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7261 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7321 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     7381 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7441 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7501 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7561 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7621 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7681 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7741 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7801 agttttcgtt ccactgagcg tcagacccc
//
